Fig. 3
Mettl3/IGF2BP1-mediated m6A modification is involved in the upregulation of LNCAROD. A Mettl3 expression in HCC was analyzed using the TCGA database. B RIP-RT-qPCR assay results show the enrichment of m6A-modified LNCAROD in SNU-449 and HCC-LM3 cells compared with THLE2 cells. C-D Effect of Mettl3 knockdown or overexpression on LNCAROD expression. E RIP-RT-qPCR results show the enrichment of m6A-modified LNCAROD following Mettl3 depletion. F LNCAROD stability analysis in HCC-LM3 cells with Mettl3 silencing or overexpression in the presence of actinomycin D. G RIP-RT-qPCR assay results show the enrichment of the insulin-like growth factor 2 mRNA-binding protein 1 (IGF2BP1) on LNCAROD in HCC cells. H-I Effect of IGF2BP1 knockdown or overexpression on LNCAROD expression. J LNCAROD stability analysis in HCC-LM3 cells with IGF2BP1 silencing or overexpression in the presence of actinomycin D. K RIP-RT-qPCR assay results show the enrichment of IGF2BP1 on LNCAROD upon Mettl3 silencing. Data are shown as the mean ± SD. *P < 0.05; **P < 0.01