Fig. 5

Implication of SRSF3 in LNCAROD-mediated regulation of PKM alternative splicing and HCC malignancy. A FISH assay determining the location of LNCAROD in HCC cells. B RT-qPCR assay of the nuclear and cytoplasmic distribution of LNCAROD in HCC cell lines. C RIP-RT-qPCR assay showing the relative enrichment of LNCAROD in anti-IgG- or anti-SRSF3-specific immunoprecipitate. D RNA pull-down assay was used to detect the interaction between LNCAROD and SRSF3. E-F Effect of SRSF3 knockdown on the LNCAROD-mediated PKM1 and PKM2 alterations in SMMC-7721 and Huh7 cells at mRNA and protein levels. Effect of SRSF3 knockdown on LNCAROD-mediated increase of Huh7 cell proliferation (G-H) (G cell viability; H colony formation), migration and invasion (I), glucose consumption (J), lactate production (K), ATP production (L), and extracellular acidification rate (ECAR) (M). Data are shown as the mean ± SD. ^*P < 0.05; ^**P < 0.01