Fig. 6

miR-145-5p sponging activity inhibits PKM2 and is involved in the oncogenic roles of LNCAROD in HCC. A Dual luciferase reporter assay determined the relative luciferase activities in HCC cells following the indicated transfection. B Effect of LNCAROD overexpression on miR-145-5p level in HCC cells. C RIP assay evaluated the relative enrichment of LNCAROD and miR-145-5p in anti-IgG- or anti-AGO2-specific immunoprecipitate. D RNA pull-down assay was used to detect the interaction among LNCAROD, miR-145-5p, and AGO2. E Dual luciferase reporter assay determined the relative luciferase activities in HCC cells following the indicated transfection. F-G Alteration of PKM1/PKM2 levels after the overexpression of miR-145-5p. H-I Effect of miR-145-5p on LNCAROD mediated alteration of PKM2 levels. Data are shown as the mean ± SD. ^*P < 0.05; ^**P < 0.01