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Fig. 1 | Journal of Experimental & Clinical Cancer Research

Fig. 1

From: TAGLN mediated stiffness-regulated ovarian cancer progression via RhoA/ROCK pathway

Fig. 1

Ovarian cancer metastases exert higher stiffness than primary tumors. A The Young’s modulus of paired primary tumors and omental metastases of HGSOC assessed by AFM analysis. Data are relative average compressive Young’s modulus, analyzed from eight paired samples per condition. B Representative immunofluorescence images (left) and quantification (right) for p-FAK (Y397) (green, left) and p-MLC2 (green, middle) with DAPI (blue) for paired tumors of HGSOC (n = 3, *P < 0.05). Scale bar: 50 μm. C Trichrome staining (left) of paired tumors of HGSOC and quantification (right) (n = 8). Scale bar: 50 μm. D Photomicrographs of paired tumors of HGSOC in stained with picrosirius red, viewed under orthogonal polarizing filters (left) and quantification (right) (n = 8). Scale bar: 50 μm. E Immunohistochemistry (IHC) assess of collagen I in paired tumors of HGSOC (left: representative images of IHC staining, right: quantification of collagen I expression, n = 8). Scale bar: 20 μm. F Boxplots showing the expression level of average major fibrillar collagen signature in paired (left) or unpaired (right) primary tumors and metastases of ovarian cancer included in ovarian dataset. G Immunohistochemistry (IHC) assess of LOX in paired tumors of HGSOC (left: representative images of IHC staining, right: quantification of LOX expression, n = 8). Scale bar: 20 μm. H Boxplots showing the expression level of LOX in paired (left) or unpaired (right) primary tumors and metastases of ovarian cancer included in ovarian dataset

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