Fig. 2

Activin-A represses the expression of inhibitory receptors on lung tumor-infiltrating CD4⁺ T cells. A Representative flow cytometry plots (left) and cumulative median fluorescence intensity (MFI) values of PD-1, CTLA-4 or LAG-3 expression by CD3⁺CD4⁺ T cells infiltrating lung tumors (right) from activin-A-treated or untreated LLC-OVA-tumor bearing mice. Numbers in plots indicate MFI values. Shaded histograms represent isotype controls. B Representative flow cytometry plots (left) and cumulative percentages of Foxp3-expressing cells among CD3⁺CD4⁺ T cells infiltrating lung tumors (right). C Representative flow cytometry plots (left) and cumulative percentages of IL-10-expressing cells among CD3⁺CD4⁺ T cells infiltrating lung tumors (right). D Representative flow cytometry plots (left) and cumulative percentages of IFN-γ-expressing cells among CD3⁺CD4⁺ T cells infiltrating lung tumors (right). Data are representative of 6 independent experiments. Statistical significance was obtained by unpaired Student’s t-test; **P < 0.01 and ***P < 0.001