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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: ZNF507 affects TGF-β signaling via TGFBR1 and MAP3K8 activation in the progression of prostate cancer to an aggressive state

Fig. 2

Decreased cancer properties, including cell proliferation, colony formation, and metastasis from ZNF507 knockdown in PC cells. A, B Relative ZNF507 mRNA levels from shRNA-mediated knockdown compared with the scramble control in DU145 and 22Rv1 cells. Each cell lines were prepared for three independent experiments with at least three samples per group. C Representative protein expression data assessed by western blot for ZNF507 in scramble or shZNF507 DU145 or 22Rv1 cells; shZNF507 #5 treated DU145 and 22Rv1 cells. D, E CCK-8 proliferation assay of the scramble or shZNF507 DU145 or 22Rv1 cells. The optical density (OD) value at 450 nm was determined. Each experiment consists of six samples from each group of cells were performed. Three independent experiments were performed. F Colony formation of the shZNF507 treated DU145 or 22Rv1 cells performed by soft agar assay. G Statistical analysis of colony number and relative diameter analyzed from the colony formation assay data. Each experiment consists of four samples from the same group of cells were measured and the three independent experiments were conducted. H PCNA staining of the scramble or shZNF507 DU145 or 22Rv1 cells performed by immunocytochemistry (Scale bar = 50 μm). The graph in the right panel presents relative corrected total cell fluorescence (CTCF). At least 4 pictures from each sample were taken and the CTCF were calculated. I Representative images of the invasion and migration assay performed with the scramble or shZNF507 DU145 or 22Rv1 cells using trans-well plate (Scale bar = 50 μm). J Relative ratio of migration and invasion of the data from the transwell migration and invasion assay. At least four pictures from each sample were taken and the three independent experiments were performed. K, L Relative mRNA expression of Slug, Twist1, Snail, KLF8, ZEB1, and ZEB2 in the scramble or shZNF507 DU145 or 22Rv1 cells analyzed by qRT-PCR. GAPDH was used as a normalization control. The three independent experiments consist of at least four samples per group were performed. M Representative images of protein expression data of E-cadherin, N-cadherin, Vimentin, Snail, and Slug in the scramble or shZNF507 DU145 or 22Rv1 cells assessed by western blot. β-actin was used as an endogenous control. The data are presented as the Means ± SD from three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001 versus scramble control

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