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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: ZNF507 affects TGF-β signaling via TGFBR1 and MAP3K8 activation in the progression of prostate cancer to an aggressive state

Fig. 3

ZNF507 knockdown induce cell cycle arrest and apoptosis in PC cells. A Cell cycle analysis was performed with scramble or shZNF507 DU145 or 22Rv1 cells by propidium iodide (PI) staining. The proportion of cells in each cycle was measured. B Ratio of cell cycle population from cell cycle analysis. Three independent experiments were performed and at least four samples per group were measured in each experiment. C, D Relative mRNA expression of CDK1, CDK2, CDK4, CDK6, CyclinA1, CyclinD1, CyclinE1, and CDC25a in the scramble or shZNF507 DU145 or 22Rv1 cells assessed by qRT-PCR. GAPDH was used as normalization control. The data are presented as the Means ± SD from three independent experiments. E Representative images of the protein expression data of CyclinA1, CyclinB1, CyclinD1, CyclinE1, CDK2, CDK4, and CDK6 in the scramble or shZNF507 DU145 or 22Rv1 cells assessed by western blot. β-actin was used as an endogenous control. F Apoptosis analysis conducted by PI-Annexin V-FITC staining in the scramble or shZNF507 DU145 or 22Rv1 cells. G Graph of cell population ratio from the apoptosis analysis of the scramble or shZNF507 DU145 or 22Rv1 cells. Three independent experiments were performed and at least 4 samples per group were measured in each experiment. H Representative images of the protein expression data of Bax, Survivin, Bcl-xL, Bcl-2, Caspase3, cleaved-Caspase3, PARP, and cleaved-PARP in the scramble or shZNF507 DU145 or 22Rv1 cells assessed by western blot. β-actin was used as an endogenous control. For all the data, at least three independent experiments were performed. *p < 0.05, **p < 0.01, ***p < 0.001 versus scramble control

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