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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: ZNF507 affects TGF-β signaling via TGFBR1 and MAP3K8 activation in the progression of prostate cancer to an aggressive state

Fig. 4

Knockdown of ZNF507 in PC cells displays altered TGF-β signaling. A Heat map of differentially expressed gene values in shZNF507 cells compared with their respective scramble groups, as performed by microarray analysis. Red arrows indicate the state of ZNF507, TGFBR1, MAP3K8, and FURIN. B Venn diagram describing the number of differentially expressed genes in DU145 and 22Rv1 experiments. Font in red indicates the number of up-regulated genes, blue indicates the number of down-regulated genes, and green indicates the number of inversely-regulated genes in both groups. C, D KEGG pathway enrichment analysis in the shZNF507 DU145 or 22Rv1 cells compared with their respective scramble groups. E, F Relative TGFBR1, MAP3K8, and FURIN mRNA expression assessed by qRT-PCR analysis of the shZNF507 DU145 or 22Rv1 cells compared with their respective scramble cells. GAPDH was used as an endogenous normalization control. The data are presented as the Means ± SD from three independent experiments. G Representative images of the protein expression data of TGFBR1, MAP3K8, and FURIN detected by western blot in the scramble and shZNF507 #5 DU145 or 22Rv1 cells. H Representative images of the protein expression data of canonical TGF-β signal proteins, Smad2, and Smad3 with their phosphorylated forms, assessed by western blot. β-actin was used as a normalization control. I Representative images of the protein expression data of non-canonical TGF-β signal proteins, RAS, MEK, phosphorylated-MEK, ERK, and phosphorylated-ERK, examined by western blot. β-actin was used as a normalization control. J Schematic of TGF-β signal pathway in PC and related factors (red framed). For all the data, at least three independent experiments were performed. **p< 0.01, ***p < 0.001 versus scramble control

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