Fig. 5

ZNF507 as an activator of TGFBR1, MAP3K8, and FURIN transcription, and their positive correlation in PC. A Spearman correlation analysis of ZNF507 and TGFBR1 levels compared to the Gapdh levels in PC samples based on the GEPIA database (TPM, Transcripts per Kilobase Million, n = 472). B Spearman correlation analysis of ZNF507 and MAP3K8 expression normalized to the Gapdh levels in PC based on the GEPIA database (n = 472). C Spearman correlation analysis of ZNF507 and FURIN normalized to the Gapdh levels in PC from the GEPIA database (n = 472). D Representative protein expression data of ZNF507, TGFBR1, MAP3K8, and FURIN in the benign hyperplasia and PC tissue (Gleason score over 8) specimens assessed by western blot. β-actin was used as a normalization control. E Average of relative protein expression of ZNF507, TGFBR1, MAP3K8, and FURIN compared with the intensity of β-actin in the benign hyperplasia (n = 6) and PC tissue (Gleason score over 8m n = 15) specimens. Three independent experiments were performed and the average rate of each sample were analyzed. F Relative mRNA expression of ZNF507, TGFBR1, MAP3K8, and FURIN determined by qRT-PCR analysis of the ZNF507 overexpression (ZNF507 OE) DU145 cells compared to the mock control.G Representative images of the protein levels of ZNF507, TGFBR1, MAP3K8, and FURIN in the mock and ZNF507 OE DU145 cells examined by western blot. β-actin was used as a normalization control. H, I Schematics of TGFBR1 and MAP3K8 promoter regions and related four sets of ChIP qRT-PCR primers. J Dual reporter luciferase assay conducted with pGL3-Basic or pGL3 containing TGFBR1 promoter vectors and pcDNA3.1-Mock or pcDNA3.1-ZNF507 vectors. K Dual reporter luciferase assay conducted with pGL3-Basic or pGL3 containing MAP3K8 promoter vectors and pcDNA3.1-Mock or pcDNA3.1-ZNF507 vectors. L qRT-PCR analysis of TGFBR1 promoter regions conducted with 4 sets of specific primers after a ChIP assay with DU145 cells, with anti-ZNF507 antibody or the IgG isotype control. The results presented are fold enrichments relative to IgG control. M qRT-PCR analysis of MAP3K8 promoter regions conducted with 3 sets of specific primers after a ChIP assay in DU145 cells using an anti-ZNF507 antibody to query binding, with IgG used as an antibody control. The results presented are fold enrichment relative to the IgG control. Data are presented as the Means ± SD from three independent experiments including at least 3 independents samples. *p < 0.05, **p < 0.01, ***p < 0.001 versus normal tissue or Mock control