Fig. 3

circHMGCS1–016 functions as a sponge for miR-1236-3p. A. CircRIP was performed in QBC939 cells using circHMGCS1–016 and negative control probes, and we found a specific enrichment of circHMGCS1–016 and miR-1236-3p compared with the negative control; B. Putative binding sites of miR-1236-3p with respect to circHMGCS1–016 were predicated via StarBase v3.0. C. The luciferase activity of pLG3-circHMGCS1–016 in the 293 T cells after co-transfection with miR-1236-3p. Data are representative of 3 independent tests (*** p < 0.001, n.s. p > 0.05); D. The level of circHMGCS1–016 in the streptavidin-captured fractions of the QBC939 cell lysates after transfection with biotinylated miR-1236-3p or the negative control (NC). Data are representative of 3 independent tests (*** p < 0.001, n.s. p > 0.05); E. FISH analysis in QBC939 cells showed that circHMGCS1–016 was colocalized with miR-1236-3p in the cytoplasm; F. The relative level of miR-1236-3p was measured by qRT-PCR in the ICC cells; Data are representative of 3 independent tests; G. The expression of miR-1236-3p was modified in ICC cells; Data are representative of 3 independent tests (*** p < 0.001); H. The interference of miR-1236-3p in QBC939-shcircHMGCS1–016 and RBE-control cells up-regulated the invasive ability of ICC cells (Bar = 200 μm); Data are representative of 3 independent tests(*** p < 0.001); I. The interference of miR-1236-3p in QBC939-shcircHMGCS1–016 and RBE-control cells up-regulated the ability of colony formation; Data are representative of 3 independent tests (** p < 0.01, *** p < 0.001); J. CCK-8 assay showed that the interference of miR-1236-3p in QBC939-shcircHMGCS1–016 and RBE-control cells up-regulated the ability of cell proliferation. Data are representative of 3 independent tests (** p < 0.01, *** p < 0.001)