Fig. 4

FLVCR1-AS1 functions as a miR-513c-5p and miR-514b-5p sponge in PC. A Relative FLVCR1-AS1 expression in subcellular localization predicted by lncLocator. B Representative FISH images showing the expression of FLVCR1-AS1 in PANC-1 and PATU-8988 cells (RED). The nucleus was stained using DAPI (BLUE). C Relative FLVCR1-AS1 expression levels in subcellular fractions. D The expression of six potential target miRNAs in PANC-1 and PATU-8988 cells after FLVCR1-AS1 overexpression. E Promoter luciferase activity of miR-513c-5p and miR-514b-5p in 293-T cells overexpressing FLVCR1-AS1. F The expression of pri-miR-513c-5p and pri-miR-514b-5p in PANC-1 cells transfected with empty control or pcDNA-FLVCR1-AS1. G The expression of pre-miR-513c-5p and pre-miR-514b-5p in PANC-1 cells transfected with empty control or pcDNA-FLVCR1-AS1. H RIP assay was performed using rabbit AGO2 and IgG antibodies in PANC-1 cells. Relative expression levels of FLVCR1-AS1, miR-513c-5p, and miR-514b-5p were determined by qRT-PCR. I Luciferase activity in 293-T cells co-transfected with FLVCR1-AS1 wild-type or mutant sequence and miR-513c-5p or miR-514b-5p mimics. Scale bar = 20 μm. **P < 0.01; ns, no significance. All experiments were repeated three times