Fig. 5
miR-513c-5p and miR-514b-5p are upregulated in PC and are associated with poor prognosis and promote proliferation and migration in PC cells. A miR-513c-5p and miR-514b-5p expression in 77 PC tissues compared to matched normal tissues. B The correlation between FLVCR1-AS1 and miR-513c-5p and miR-514b-5p expression in 77 PC tissue samples. C Prognostic analysis of miR-513c-5p and miR-514b-5p using survival data of 51 patients from our center. D CCK-8 assays showed that FLVCR1-AS1 overexpression inhibits PANC-1 cell proliferation. Co-transfection of miR-513c-5p or miR-514b-5p mimics and pcDNA-FLVCR1-AS1 eliminated the decrease in proliferation induced by FLVCR1-AS1 overexpression. E EdU assays showed that FLVCR1-AS1 overexpression inhibits PANC-1 cell proliferation. miR-513c-5p or miR-514b-5p overexpression promotes PANC-1 cell proliferation. Co-transfection of miR-513c-5p or miR-514b-5p mimics with pcDNA-FLVCR1-AS1 eliminated the decrease in proliferation rates. F-G Wound healing and Transwell assays showed that FLVCR1-AS1 overexpression decreases PANC-1 cell migration, while miR-513c-5p or miR-514b-5p overexpression promotes PANC-1 cell migration. Co-transfection of miR-513c-5p or miR-514b-5p mimics with pcDNA-FLVCR1-AS1 abolished the decrease in migration ability in PANC-1 cells. Scale bar = 50 μm *P < 0.05; **P < 0.01; ***P < 0.001; ns, no significance. All experiments were repeated three times