Fig. 4
Genes upregulated in BTSH indicates favourable prognosis and trastuzumab response in HER2+ patients. A Representation of differential gene expression values from microarrays, qRT-PCR validation and the role as prognosis and predictive biomarkers of “inverse amount” genes found in BTRH and BTSH versus parental BT474 cell line. White squares mean no validation. B Kaplan-Meier analysis of the expression of the indicated genes and its correlation with relapse free survival in HER2+ breast cancer patients using the KM plotter bioinformatic tool. The chart below was performed using the multigene classifier tool by selecting the combined gene expression of the four indicated genes. The cut-off value to split patients into low or high expression were lower and upper quartiles. RFS: relapse free survival. C Box-plots of genes validated for trastuzumab response in HER2+ breast cancer patients using the pathological complete response database in ROC plotter. Graphs show normalized gene expression in trastuzumab non-responders and responders patients. Gene combinations analysis was assessed by selecting the option signature