Fig. 2

dPSA is co-localized with nucleolin on the cell surface and inside SK-MEL-28 human melanoma cells. dPSA and nucleolin were identified on the surface (no detergent) or inside (+Triton X-100) SK-MEL-28 cells with anti-dPSA mAb SEAM 2 or anti-nucleolin mAb MS-3, respectively, and detected by fluorescently labeling with Alexa Fluor 594 (red fluorescence) or Alexa Fluor 488 (green fluorescence)-conjugated anti-mouse subtype-specific antibodies using confocal laser scanning microscopy. Blue fluorescence is DAPI DNA staining. A Cells with irrelevant murine IgG1 and IgG3 and secondary antibodies and (B) cells with SEAM 2 and MS-3; the arrows in merge image indicate dPSA concentrated in lamellipodia, podosomes, and filopodia in “No detergent” micrographs. Arrows in merge image of +Triton X-100-treated cells show nuclear nucleolin, which is not marked by mAb SEAM 2. Scale bars, 20 μm