Fig. 1
Alloantigen-activated CD4+ (AAA-CD4+) T cells can eliminate pre-established melanoma in mice. a-b: CD8+ and CD4+ T cells isolated from the spleens of BALB/c mice were separately activated in mixed lymphocyte cultures with B6-derived Flt3L-DCs pulsed with human gp100 peptides and the lysate of B16F1, respectively. Host B6 mice were subcutaneously injected with 1 × 106 B16F1 cells on day zero. Nine days after B16F1 inoculation, the activated CD8+ T cells or activated CD4+ T cells (AAA-CD4+ T cells) were separately injected intratumorally into the host B6 mice. The PBS-injected group served as the control. Tumor growth (a) and survival (b) are shown. c-e: AAA-CD4+ T cells were produced from BALB/c mouse CD4+ T cells in cultures activated by either B6-derived Flt3L-DCs or GM-DCs. Host mice were subcutaneously injected with 1 × 106 B16F1 cells on day zero. Nine days after B16F1 inoculation, AAA-CD4+ T cells were intratumorally injected. Tumor growth (c) and survival (d) are shown. Percent changes in BW of mice in the GM-DC-induced AAA-CD4+ and PBS-injected control groups are shown (e). Data are expressed as the mean ± SD. **P < 0.01. Abbreviations: N.S.: not significant