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Fig. 6 | Journal of Experimental & Clinical Cancer Research

Fig. 6

From: Alloantigen-activated (AAA) CD4+ T cells reinvigorate host endogenous T cell immunity to eliminate pre-established tumors in mice

Fig. 6

AAA-CD4+ T-cell therapy increases tumor infiltration of host-activated DCs and activated macrophages. AAA-CD4+ T cells were produced from BALB/c mouse CD4+ T cells in cultures activated with B6-derived GM-DCs. Host B6 mice were subcutaneously injected with 1 × 106 B16F1 cells on day zero. Nine days after B16F1 inoculation, AAA-CD4+ T cells were intratumorally injected (AAA-CD4+ group). B6-derived DC-activated B6-derived CD4+ T cells (auto-CD4+ group) and PBS-injected mice were used as controls (three mice per group). a: Representative confocal images of the indicated markers in tumors isolated 24 h after immunotherapy are shown. Bar, 20 μm. b: Graph shows the percentage of I-A/I-E high proportion of host CD11c+/F4/80DCs at 4 h after therapy. c: Graph showing the number of host CD11c+/MHC-IIhi/F4/80 DCs that infiltrated the tumor. d: Representative histogram showing the percentage expression of the indicated co-stimulatory molecules on the host CD11c+/MHC-IIhi/F4/80 DCs in the AAA-CD4+ T cell group at 24 h after therapy. e: The number of host F4/80+ macrophages that infiltrated the tumor is shown. f: Relative expression levels of the indicated genes in the tumor-infiltrated host F4/80+ macrophages in each group at 24 h after therapy, normalized to those in PBS-treated mice. Data are expressed as the mean ± SD. **P < 0.01, ***P < 0.001. Representative data from two independent experiments are shown. Abbreviations: TV: tumor volume

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