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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: PFKFB4 is overexpressed in clear-cell renal cell carcinoma promoting pentose phosphate pathway that mediates Sunitinib resistance

Fig. 5

PFKFB4 is associated with pentose phosphate pathway (PPP) in ccRCC. A) from the Cancer Genome Atlas (TCGA) clear-cell renal cell carcinoma (KIRC) dataset, shown was functional annotation of gene enriched in PFKFB4-overexpressed cases (z-score of > 2 in RNA-seq samples) analyzed using NET-GE; B) Heatmap showing metabolites significantly changed in 786O cells with PFKFB4-knockdown (KD) versus negative control (NC); C) Metabolic Set Enrichment Analysis (MSEA) of 786O cells with PFKFB4-KD over NC; D) Box plots of individual pentose phosphate pathway metabolites that were significantly changed in 786O cells with PFKFB4-KD or NC; E) Glucose uptake and lactate secretion measured in 786O cells with PFKFB4-KD or NC; F) Intact cellular respiration measured using live cell real-time metabolism monitoring under basal conditions or in the presence of FCCP in786O cells with PFKFB4-KD or NC; G) Glucose oxidation measured by 14 C-CO2 production in 786O cells with PFKFB4-KD or NC following 3-hour labeling with D[U-14 C]glucose; H) Fatty acid oxidation measured by 14 C-CO2 production in 786O cells with PFKFB4-KD or NC following 3-hour labeling with [U-14 C]palmitate. (All in vitro assays performed in triplicates and at least 3 biological replicates; Student’s t-test for all comparisons; N = 4; ns = not significant; *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001)

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