Fig. 4

A In vitro cytotoxicity of Au NR@PAMAM-GX1 on HCT-8 cells and L929 cells over an evaluation period of 24 h. B Cell viability of HCT-8 cells pretreated by Au NR@PAMAM-GX1 with different concentrations with and without NIR irradiation. C CCK-8 assay of HCT-8 cells viability after treatment with the Au NR@PAMAM-GX1or Au NR@PAMAM -GX1/FAM172A polyplexes (N/P = 40: 1, 1 μg FAM172A per well) for 24 h, followed by laser irradiation for 5 min (1: PBS; 2: Au NR@PAMAM-GX1; 3: Au NR@PAMAM-GX1/FAM172A). D Images of PI and Calcein-AM double-stained HCT-8 cells under different treatments. E Flow cytometry analysis of HCT-8 cell apoptosis in different treatment groups