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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: MYSM1 inhibits human colorectal cancer tumorigenesis by activating miR-200 family members/CDH1 and blocking PI3K/AKT signaling

Fig. 3

MYSM1 inhibits CRC cell growth in vitro. A Analysis of MYSM1 knockdown or overexpression efficiency at the mRNA level (upper) and protein level (lower). The data are shown as the means ± SDs (***P < 0.001, n = 3 independent experiments). B Cells with MYSM1 knockdown or overexpression were analyzed with a growth curve assay. The error bars indicate the SDs (*P < 0.05 and **P < 0.01, n = 6 independent experiments). C The expression and localization of MYSM1 (red) or PCNA (red) were determined by IF assay. The nuclei were counterstained with DAPI, and the significant differences in MYSM1 or PCNA staining are marked by white arrows (n = 3 independent experiments). Scale bars: 10 μm. D Western blot analysis of PCNA expression in MYSM1-knockdown HCT116 cells or MYSM1-overexpressing SW620 cells. E and F Cell cycle progression (E) and apoptosis (F) were analyzed in HCT116 cells transfected with siMYSM1/siNC and SW620 cells infected with Lenti-MYSM1/Lenti-NC by flow cytometry. The data are shown as the means ± SDs (*P < 0.05 and **P < 0.01, n = 3 independent experiments)

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