Fig. 5

MYSM1 directly binds to the promoter regions of miR-200 family members and CDH1 to initiate transcription. A and B qRT-PCR analysis of the alterations in the expression levels of 36 miRNAs following MYSM1 knockdown in HCT116 cells (A) or overexpression in SW620 cells (B). The data were logarithmically transformed (log2) and are shown as the means ± SDs (n = 3 independent experiments). The dotted line divides the data (< 1 or > 1). C-N ChIP analysis of the enrichment in the promoter regions of miR-200b-a-429, miR-200c-141 and CDH1 using IgG (Ctrl) and specific MYSM1 (C, E and K), H2AK119ub1 (D, F and L), H3K4me3 (G, I and M) and H3K27me3 (H, J and N) antibodies in untreated HCT116 and SW620 cells. The data are shown as the means ± SDs (N. S., no significance; *P < 0.05 and **P < 0.01, n = 3 independent experiments)