Fig. 2
MEST promotes invasion and migration of lung cancer cells. A, B A549 and H1299 cells were overexpressed with MEST, while A549-i8 and H1299-i8 cells were transfected with two siRNAs against MEST (si-MEST#1 and si-MEST#2, 100 nM), their invasion and migration abilities were measured by transwell assay. Scale bar, 100 μm. All data are representative of three independent experiments (C, D). Bars, S.D. *P < 0.05, **P < 0.01, ***P < 0.001 (Student’s t-test). E–F Nude mice were transplanted with luciferase-labeled cells with or without MEST overexpression (2 × 106 cells per mouse) via tail vein injection (n = 6); mice were then visualized 1 month after transplantation by using an IVIS 200 Imaging System. Lungs harvested after imaging are shown. Note that only MEST-overexpressing A549 cells formed large and more metastatic nodules in the lungs. The pulmonary metastases in the mouse model were histologically analyzed by H&E staining (F). Scale bar, 100 μm. G-I NCG mice were transplanted with the luciferase-labeled A549-i8 cells expressing two shRNA against MEST (1 × 106 cells per mouse) via tail vein injection (n = 6); the mice were visualized 1.5 months post-transplantation by using an IVIS 200 Imaging System (G). Lungs harvested after imaging are shown (H). Note that only the control group cells formed large and more metastatic nodules in the lungs. The pulmonary metastases in the mouse model were histologically analyzed by H&E staining (I). Scale bar, 100 μm