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Fig. 6 | Journal of Experimental & Clinical Cancer Research

Fig. 6

From: Tumor cell-derived SPON2 promotes M2-polarized tumor-associated macrophage infiltration and cancer progression by activating PYK2 in CRC

Fig. 6

SPON2 promotes monocyte transendothelial migration and tumor growth by activating integrinβ1/PYK2 axis. a GSEA of the focal adhesion signature and integrin pathway signatures in the SPON2-high group compared to the SPON2-low group. NES, normalized enrichment score. b Q-RT-PCR showed the mRNA expression of PYK2 and FAK in CRC cell lines (SW480 and SW620) and a mononuclear cell line (THP-1). c Western blot analysis showed the protein expression of PYK2 and FAK in CRC cell lines (SW480 and SW620) and a mononuclear cell line (THP-1). d THP-1 cells were treated with conditioned medium from stable cell lines as indicated, and the expression of representative molecules in the focal adhesion pathway was analyzed by western blotting. e Expression and colocalization of PYK2 and zyxin in THP-1 cells with different treatments by immunofluorescence (IF) staining. Scale bars, 10 μm. f Transendothelial migration of THP-1 toward conditioned medium from stable cell lines with different treatments. Scale bar, 100 µm. g Gross images of different treatment subcutaneous tumor groups, including MC38/Scramble, MC38/shSpon2#1, MC38/shSpon2#2, anti-integrin β1 and defactinib. h Growth curve in the different treatment groups. Two-way ANOVA test, **p < 0.01, ***p < 0.001. i Treatment time diagram and fold change of tumor volume in each case (final volume / initiate volume). Data represent mean ± SD, Student’s t test, *p < 0.05, **p < 0.01, ****p < 0.0001

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