Fig. 2
Knockdown of INMT suppresses CRPC development. A Western blot for the expression of indicated protein in INMT-KD and control Myc-CaP cells. B MTT assay for the proliferation rates of INMT-KD and control Myc-CaP cells in charcoal-treated medium. C-E Allograft tumor development in castrated FVB male mice inoculated with 5 × 105 INMT-KD or control Myc-CaP cells (C). Thirty seven days later mice were sacrificed and tumor were collected and weighted (D), and the expression of indicated protein in tumors was analyzed by western blot (E). F Western blot for the expression of indicated protein in INMT-KD and control DU145 cells. G MTT assay for the proliferation rates of INMT-KD and control DU145 cells. H-J Xenograft tumor development in RAG1−/− male mice inoculated with 3 × 106 INMT-KD or control DU145 cells (H). Forty two days later mice were sacrificed and tumor were collected and weighted (I), and the expression of indicated protein in tumors was analyzed by western blot (J). *, P < 0.05; **, P < 0.01