Fig. 8

In vivo role of DNMT1/miR-378a-3p/TRAF1/p65 in regulating HCC angiogenesis. A-C Tumor growth of mice subcutaneously implanted with treated HCCLM3 cells. Tumor volume and weight were measured, and tumor size is shown. D Immunohistochemical staining of CD34, DNMT1, TRAF1, p65 and VEGF in the tumors from subcutaneously implanted mice. E FISH assay for miR-378a-3p and immunohistochemical staining for DNMT1, TRAF1, p65, VEGF and CD34 were performed on 10 human HCC specimens. F A working model showing how miR-378a-3p inhibits HCC tumor angiogenesis by inactivating the NF-κB signaling pathway. *P < 0.05; **P < 0.01; ***P < 0.001