Fig. 6

SRC is involved in L1CAM-dependent STAT3 phosphorylation. (A) SFE assay was conducted on Ov90-mock and Ov90-L1CAM upon treatment with 20 μM JAK inhibitor (JAKi). (B) Immunoblots for phosphorylated or total SRC on serum-starved Ov90-mock and Ov90-L1CAM cells. α-tubulin served as loading control. (C) Second-generation spheres from Ov90-mock and Ov90-L1CAM cells were lysed and immunoblotted for phospho-STAT3, total STAT3, phospho-SRC and total SRC. Vinculin served as loading control. (D) SFE assay on Ov90-mock and Ov90-L1CAM cells upon SU6656 (SRCi) treatment. For each analysis, data are expressed as means ± SEM from three independent experiments. Comparisons between experimental groups were done with two-sided Student’s t-tests; **p < 0.01; ns = not significant