Fig. 6
From: Arachidonic acid drives adaptive responses to chemotherapy-induced stress in malignant mesothelioma
Arachidonic acid and not its downstream metabolites, mediated the early increase of ALDHbright cells and the NFkB activation. A. Representative graphs showing the percentage of NCI-H2373 ALDHbright cells detected by FACS analysis after addition of ctrl or AA, in absence or presence of pretreatment with indomethacin (10 micromol/L) and A-64077 (Zileuton) (5 micromol/L), 90 min before addition of AA. Left panel; percentage of ALDHbright cells detected 24 h after addition of the indicated drugs. Right panel: percentage of ALDHbright cells detected 72 h after addition of the indicated drugs. B. Representative graphs showing the levels of p65 NFkB detected by ELISA in the NCI-H2373 and HP1 MPM cells treated for 24 h as indicated in 6A. Statistics: * p < 0.05 ** p < 0.01 ns = not significant (p > 0.05)