Fig. 7
From: Arachidonic acid drives adaptive responses to chemotherapy-induced stress in malignant mesothelioma
Arachidonic acid modulated the sphere forming ability of MPM cells. Briefly, MSTO-211H and HP1 cells were clonally seeded in presence or absence of AA (3micromol/L) and allowed to grow for 7 days. A. Graphs showing quantitation of the sphere forming efficiency (SFE) from MSTO-211H (left panel) and HP1 (right panel) cells treated with ctrl or AA, at different seeding densities. Statistics: * p < 0.05 ** p < 0.01 ns = not significant (p > 0.05). B. Upper panel: representative micrographs of the formed spheroids (HP1, left; MSTO-211H, right) at day 4 from ctrl - and AA -treated cultures. Scale bar: 200 μm. Lower panel: graphs reporting the average (longer) diameter of the HP1 and MSTO-211H-derived spheroids in time. Statistics: * p < 0.05 ** p < 0.01 ns = not significant (p > 0.05)