Fig. 7
From: An SETD1A/Wnt/β-catenin feedback loop promotes NSCLC development
SETD1A is a direct target of the Wnt/β-catenin pathway. A, Schematic showing the putative TCF/LEF binding site in the TCF7L2/TCF4 enriched region. Red line, putative TCF/LEF binding site predicted by PROMO online tool; green region, TCF7L2/TCF4 enriched region derived from ENCODE database. B, The enrichment of β-catenin and TCF4 in the SETD1A promoter region was detected by ChIP-PCR assay. C, The enrichment of β-catenin and TCF4 in the SETD1A promoter region was detected by ChIP-qPCR assay. D, The promoter activity of SETD1A gene in NSCLC cells was analyzed by luciferase activity assay. ns, not significant. E, SETD1A transcript levels in NSCLC cell as indicated were detected by qRT-PCR. H, SETD1A protein levels in NSCLC cells as indicated were detected by western blotting. Data are shown as the means ± SD. *P < 0.05, **P < 0.01.