Fig. 1

High expression levels of HOTAIRM1 in NPM1-mutated AML cells. a Heat maps of the top 30 lncRNAs upregulated in NPM1-mutated AML samples compared to NPM1-wt AML samples (left, GSE15434; right, TCGA). b Volcano plots showing the expression profiles of lncRNAs (top, GSE15434; bottom, TCGA). c HOTAIRM1 expression levels in patients with NPM1-wt AML (n = 113) and patients with NPM1-mutated AML (n = 138) were obtained from the GSE15434 dataset. d HOTAIRM1 expression levels in AML patients without (n = 118) and with NPM1 mutation (n = 33) were obtained from the TCGA database. e The expression levels of HOTAIRM1 in primary AML blasts without (n = 20) and with NPM1 mutation (n = 14). f qRT-PCR was conducted to determine the level of HOTAIRM1 in AML cell lines. GAPDH was used as the endogenous control. g-h Survival analysis based on HOTAIRM1 expression in AML (g) and NPM1-mutated AML samples (h) using the data from TCGA and BeatAML. The data are presented as the mean ± SD of three independent experiments. ***P < 0.001