Fig. 6

Nuclear HOTAIRM1 promotes leukemia cell autophagy and proliferation through EGR1. a Western blot analysis of LC3-II, p62 and EGR1 levels in OCI-AML3 cells transduced with the EGR1 expression vector. b CCK-8 assays were applied to test the proliferation of transfected OCI-AML3 cells. c-d Flow cytometry was performed to assess the cell cycle (c) and apoptosis (d) in transfected OCI-AML3 cells. e, g Western blot analysis of LC3-II, p62 and EGR1 levels in HOTAIRM1-silenced OCI-AML3 cells after EGR1 knockdown (e) and in HOTAIRM1-overexpressing OCI-AML2 cells after EGR1 overexpression (g). f, h CCK-8 assays were used to analyze cell proliferation in transfected OCI-AML3 (f) and OCI-AML2 cells (h). The data are presented as the mean ± SD of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001. n.s. indicates no significant difference