Fig. 3

PARGi suppresses mitotic entry in sensitive cell lines. A Cell fate profiling, showing cell behaviour over 115 h treatment with 1 μM PARGi or DMSO (Control). Each horizontal line represents a single cell, with the colours indicating cell behaviour. Following mitosis, one daughter cell was chosen at random to continue the analysis. No mitotic entry does not include death in interphase where mitosis does not take place. Abnormal mitosis includes cell division abnormalities such as slippage (where cells enter mitosis, but exit without division), fusion (where daughter cells appear to separate but subsequently join back together), tripolar cell divisions and division of binuclear cells. B The % of total normal mitoses in PARGi-treated compared with DMSO controls. Mean of ≥2 biological replicates. Statistics: total normal mitoses in DMSO-treated compared with PARGi-treated cells was compared by 2-way ANOVA with Sidak post-hoc test. Error bars represent SEM. ***p < 0.001, ****p < 0.0001