Fig. 4

HDAC1 or HDAC2 increases local tumor growth in vivo. Evaluation of tumorigenicity of CRISPR/Cas9 knockouts of HDAC1 or HDAC2 and their controls (Cas9) in EwS cell lines EW7 and SK-N-MC. a Immune deficient Rag2^−/−γ_C^−/−mice were injected s.c. with 4 × 10⁶ EwS cells. Kaplan-Meier plots of individual experiments with four to six mice per group are shown. Log-rank test was used to test for differences in survival. b Tumors of sacrificed mice were analyzed for cleaved caspase 3 by immunohistochemistry. Representative results of EW7 and SK-N-MC tumors are shown (10x original magnification). c Left, the number of mitoses per 10 high power fields per tumor is given. Right, level of cleaved caspase 3 in EwS tumors. The percentage of cleaved caspase 3 positive cells in five fields per tumor is given