Fig. 2

C2CD4A promoted CRC cell growth in vivo. a Image of different groups subcutaneous xenograft tumors of RKO cells was shown (five mice per group). b The volume of subcutaneous xenograft tumors were measured every week for five weeks. c The average final tumors weight was shown. d qRT-PCR and western blot were performed to detect mRNA and protein level of C2CD4A in tumors. And the protein expression levels of p53, p21, BAX, Cleaved-caspase3 and Ki-67 were shown using western blot. e IHC staining of p53, p21, BAX, Cleaved-caspase3 and Ki-67 from the indicated tumors. f Quantification of p53 expression was determined by IHC scores. Date was shown as mean ± SD with three experiments. (*P < 0.05, **P < 0.01, ***P < 0.001)