Fig. 3

VEGF₁₆₅b increases spontaneous and noxious-evoked activity of NS neurons through VEGFR-1. Representative ratematers showing spontaneous and noxious-evoked activity of NS neurons after spinal application of VEGF₁₆₅b alone or in combination with D16F7 mAb (a and b, respectively); black arrows indicate the noxious stimulation on the mouse hind-paw. Mean ± SEM population data of spinal cord application of VEGF₁₆₅b (3 ng/5 μl) in the presence of vehicle (0.9% NaCl), or D16F7 (100 pg/5 μl) on firing rate of spontaneous activity (c), frequency (d) and duration of evoked activity (e) of NS neurons in CD1 mice. Black arrows indicate vehicle, D16F7 or VEGF₁₆₅b spinal application. Each point represents the mean of 5 different mice per group (one neuron recorded per each mouse). ^#P < 0.05, ^##P < 0.01 and ^###P < 0.001 indicate statistical difference vs baseline; *P < 0.05, **P < 0.01 and ***P < 0.001 indicate statistical difference vs vehicle + VEGF₁₆₅b. One-way ANOVA followed by Dunnet’s multiple comparison post-hoc test was performed for statistical significance within groups. Two-way ANOVA followed by Bonferroni post-hoc test was used for comparison between groups