Fig. 2

TMEM44-AS1 promotes glioma cell proliferation, colony formation, migration and invasion. (A) Cell viability of LN-18 and U251 cells transfected with sh-TMEM44-AS1 or sh-con was evaluated with CCK8 assay, respectively. *P < 0.05, **P < 0.01. (B) CCK-8 assay of SF126 cells transfected with TMEM44-AS1 plasmid or vector. **P < 0.01. (C-D) Colony formation assay of LN-18 cells transfected with sh-TMEM44-AS1 or sh-con. **P < 0.01. (E-F) Colony formation assay of SF126 cells transfected with TMEM44-AS1 or empty vector. **P < 0.01. (G-J) Representative images of transwell migration and invasion assays performed in LN-18 and U251 transfected sh-TMEM44-AS1 or sh-con cells. **P < 0.01. (K-N) Representative images of transwell migration and invasion assays in SF126 cells after transfection of sh-TMEM44-AS1 or sh-con. **P < 0.01. Data are presented as mean ± SD. The independent biological experiments were repeated at least three times