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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: Super-enhancer-associated TMEM44-AS1 aggravated glioma progression by forming a positive feedback loop with Myc

Fig. 4

TMEM44-AS1 represses Myc translocation. (A-B) Subcellular fractionation analysis was performed in LN-18 and U251 cells. Cytoplasmic fraction (blue), and nuclear fraction (red) and probed for TMEM44-AS1. GAPDH was used as a cytoplasmic marker, U6 as a nuclear marker, and MALAT1 as the positive control. (C) RNA FISH assays for TMEM44-AS1. Nuclei were stained with DAPI. Scale bar = 20 μm. (D) Volcan representation of genes differentially expressed in LN-18 knockdown TMEM44-AS1 vs. LN-18 sh-con cells (green, downregulation; red, upregulation). (E) Ingenuity pathway analysis revealed that genes differentially expressed upon TMEM44-AS1 knockdown are markedly related to several cancer-associated signaling pathways. (F-G) Expression of p38MAPK and IL-6 signaling pathway associated with genes in LN-18 and U251 cells transfected with sh-TMEM44-AS1 or sh-con. *P < 0.05, **P < 0.01

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