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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: Super-enhancer-associated TMEM44-AS1 aggravated glioma progression by forming a positive feedback loop with Myc

Fig. 5

TMEM44-AS1 regulated EGR1/IL-6 and Myc signaling in glioma cells. (A-C) The expression of EGR1 upon knockdown of TMEM44-AS1 in LN-18 and U251 cells. **P < 0.01. (D-F) Expression of EGR1 in SF126 cells was examined transfected with vector or TMEM44-AS1 plasmid. **P < 0.01.(G-H) ChIP analyses of TMEM44-AS1 knockdown LN-18 and U251 cells were conducted on indicated IL-6 primer using the EGR1 antibodies. Enrichment is determined relative to input controls. *P < 0.05, **P < 0.01 vs. sh-con. (I) IL-6 expression in SF126 cells co-transfected with TMEM44-AS1 plasmid and si-EGR1. **p < 0.01 vs. vector; **P < 0.01 vs. TMEM44-AS1. (J-K) Expression of Myc protein in LN-18 and U251 cells transfected with sh-TMEM44-AS1 or sh-con. **P < 0.01. (L-N) Expression of Myc in SF126 cells transfected with vector or TMEM44-AS1 expression plasmid. **P < 0.01. Data are from three biological replicates represented as mean ± SD

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