Fig. 7
The interaction of TMEM44-AS1 and SerpinB3 activates Myc. (A) TMEM44-AS1 pulldown assay was performed and the associated proteins were separated with SDS-PAGE and silver staining. (B) SerpinB3 in TMEM44-AS1 RNA pulldown were analyzed by western blot. (C) Co-localization of TMEM44-AS1 and SerpinB3 in glioma cells. TMEM44-AS1 probes (red) and SerpinB3 antibody (green) were used for staining. Scale bars, 20 μm. (D-E) RNA immunoprecipitation (RIP) using the SerpinB3 antibody followed by RT-qPCR for TMEM44-AS1. (F-H) Expression of Myc and EGR1 in LN-18 and U251 cells co-transfected with TMEM44-AS1 plasmid and si-SerpinB3. **p < 0.01 vs. vector; **P < 0.01 vs. TMEM44-AS1