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Fig. 6 | Journal of Experimental & Clinical Cancer Research

Fig. 6

From: Differential gene expression-based connectivity mapping identified novel drug candidate and improved Temozolomide efficacy for Glioblastoma

Fig. 6

PCI-24781 decreases DNA repair machinery at the transcriptional level in GBM cells. A Cells were incubated with IC25 of TMZ, IC25 of PCI-24781 or combination for 48 h, and lysates were analyzed for RAD51, CHK1, and BRCA1 levels by immunoblotting. β-actin serves as a loading control. B Real-Time qRT-PCR for RNA expression of BRCA1, CHK1, RAD51, and Ku70 normalized to GAPDH in drug-treated (as given in (A)) U-118MG cells. The qPCR data is analyzed using the 2-ΔΔCT method. ANOVA compared the ΔCt values between groups, and Tukey’s method was used to adjust for multiple comparisons. Results are given as fold change between groups with 95% confidence intervals. ‘*’ p < 0.01; ‘#’ p ≤ 0.01.”*” significantly different from vehicle control; “#” significantly different from TMZ. Data presented as mean +/− SD, experiments done in triplicate. C Mouse syngeneic cell lines were treated with 1.5 μM PCI-24781 for 48 h, and cell lysates were analyzed for DNA repair machinery and apoptotic marker by western blotting. ‘–‘ and ‘+’ signs indicate vehicle and PCI-24781 treatment, respectively

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