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Fig. 7 | Journal of Experimental & Clinical Cancer Research

Fig. 7

From: Differential gene expression-based connectivity mapping identified novel drug candidate and improved Temozolomide efficacy for Glioblastoma

Fig. 7

PCI-24781 induced DNA DSB (γH2AX foci) in GBM cells. A GBM cells were incubated with vehicle control, TMZ, PCI-24781, and combination for 48 h and probed with γH2AX (phospho S139) antibody, and nuclei counterstained with DAPI. Representative immunofluorescence images are shown. Scale Bar 5 μm. B Quantitative measurement of γH2AX staining represented as an arithmetic mean intensity per cell. Staining data were compared between control and treatment groups using one-way ANOVA. Pairwise comparisons between control and treatment groups were adjusted for multiple comparisons with Tukey’s method. “*” significantly different compared to vehicle control; ‘*’ p < 0.05; ‘$’ p < 0.001; ‘#’ p < 0.0001. “$” significantly different compared to TMZ; “#” significantly different compared to PCI-24781. C Immunoblot analysis of γH2AX (phospho S139) levels in vehicle or drug-treated GBM cells. DNA DSB- DNA double-strand break

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