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Fig. 1 | Journal of Experimental & Clinical Cancer Research

Fig. 1

From: CircGSK3B promotes RORA expression and suppresses gastric cancer progression through the prevention of EZH2 trans-inhibition

Fig. 1

CircGSK3B is downregulated in GC cells and tissues. a Flow chart of circRNA selection. b The number of DECs against the P-value in the assessment of the 6 datasets. c Heatmap based on 14 DECs between GC and normal samples in the 6 datasets. d The genomic locus of circGSK3B. The expression level of circGSK3B was assessed via RT-PCR assay and Sanger sequencing. Arrows represent divergent primers targeting circGSK3B’s genome region (Left); RT-qPCR products using divergent primers indicating circularization of circGSK3B. cDNA represents complementary DNA. gDNA represents genomic DNA (Right). e A RT-qPCR assay was used to assess the expression of the three DECs in GES-1, SGC7901, MKN45, BGC823, AGS and MGC803 cells. f RT-qPCR analysis of circGSK3B in 56 GC and NC samples. g RT–qPCR analysis for the expression of circGSK3B and GSK3B mRNAs after treatment with Actinomycin D at the indicated time points in AGS cells. h RT–qPCR assays were used to assess the expression of circGSK3B and GSK3B mRNA in AGS cells after treatment using RNase R. Data represented as mean ± SD. Each experiment was performed five times; dot plot data points represent each independent experiment. The P-value was calculated using a two-tailed unpaired Student’s t-test. (i) The RNA-FISH assay revealed the nuclear localization of circGSK3B in MKN45 and AGS cells by an antisense probe (green). Nuclei were stained with DAPI

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