Fig. 3

Effects of circGSK3B in vivo and on cell cycle and apoptosis. a Representative images (Top left and middle), in vivo growth curve (Bottom left), and weight at the end points (Top right) of xenografts formed by subcutaneous injection of MKN45 cells stably transfected with mock, circGSK3B, sh-Scb, or sh-circGSK3B #2 into the dorsal flanks of nude mice (n = 5 for each group). b Representative images (left panel) and quantification (right panel) of immunohistochemical staining showing the expression of Ki-67 and CD31 within xenograft tumors formed by hypodermic injection of MKN45 cells stably transfected with circ-Mock or circGSK3B (n = 5 for each group). c Flow cytometry was adopted to assess the impact of circGSK3B on the cell cycle of GC cells. d Flow cytometry was used to assess the effect of circGSK3B on apoptosis in GC cells