Fig. 5

circGSK3B targeted RORA expression through EZH2-mediated epigenetic regulation in GC cells. a RT-qPCR analysis for GSK3B mRNA in AGS, MKN45 cells stably transfected with mock, circGSK3B, and sh-circGSK3B. b Overlapping analysis of DEGs between circGSK3B-OE and control group with EZH2 targets from the tftargets R package or EZH2 binding targets from Biogrid. c Volcano plot of the selected 10 DEGs in the RNAseq dataset. d RT-qPCR analysis for 10 screened mRNAs in MKN45 cells stably transfected with mock, circGSK3B, and sh-circGSK3B. e RT-qPCR analysis for RORA mRNA in AGS, MKN45 cells stably transfected with mock, circGSK3B, and circGSK3B + EZH2. f The effect of circGSK3B and EZH2 on RORA, H3K27me3, β-catenin, P-β-catenin, and CCND1 (Canonical target gene of β-catenin) assessed by western blot assay. g, h ChIP assay showing the binding of EZH2 (g) and H3K27me3 (h) to the promoter of RORA assessed in MKN45 and AGS cells stably transfected with mock, circGSK3B, or circGSK3B + EZH2