Fig. 7

Circ_0004296 inhibited PCa growth and metastasis in vivo. A, B The subcutaneous xenograft mouse model showed that overexpression of circ_0004296 inhibited PCa growth in vivo (DU145-Vector, n = 6; DU145-circ_0004296, n = 6). C H&E staining and IHC assay showed that circ_0004296 overexpression downregulated the Ki67 protein level (magnification 200×). D The orthotopic xenograft mouse model showed that overexpression of circ_0004296 suppressed PCa growth and pelvic peritoneal invasion. E IHC assay showed that circ_0004296 overexpression remarkably downregulated mesenchymal marker (Vimentin) protein level but upregulated epithelial marker (E-cadherin) protein level (magnification 200×). F IHC assay showed that overexpression of circ_0004296 remarkably reduced the ETS1 protein level (magnification 200×). G IF assay showed that the expression of EIF4A3 protein from the circ_0004296 overexpression group was increased in the nucleus as compared to that in the control group. (magnification 200×). H The in vivo tail vein injection model showed that overexpression of circ_0004296 suppressed metastasis of PCa. I Kaplan–Meier analysis showing survival of mice with intravenous xenograft (DU145-Vector, n = 8; DU145-circ_0004296, n = 5). P value was determined using a log-rank test. *P < 0.05, **P < 0.01, ***P < 0.001