Fig. 1

SGO1-AS1 is downregulated in GC tissues and associated with GC progression. a. Volcano plots of differentially expressed lncRNAs in GCs vs. matched normal tissues shown in green and red, respectively. b. SGO1-AS1 expression levels in 10 pairs of GCs and adjacent normal tissues from the GEO dataset (GSE50710). c. Relative expression levels of SGO1-AS1 in 92 paired GC and normal tissues from Cohort 2 patients were quantified by qRT-PCR. SGO1-AS1 was downregulated (> 2-fold) in 63% (58 of 92) of the GC tissues (tumor) relative to that in the adjacent noncancerous tissues (normal). d-f. Assessment of the SGO1-AS1 expression levels in GCs according to their clinical stage (d) and status of lymph node (e) or distant metastasis (f) based on a qPCR analysis of GC. g-i. RNA ISH analyses of SGO1-AS1 expression in 95 GC specimens and 80 normal tissues on tissue microarrays. Scale bar: 50 μm. j. Kaplan-Meier analyses of the overall survival of patients with GCs (n = 95) based on the SGO1-AS1 expression levels. The defined high and low expression levels of SGO1-AS1 were stratified according to the median expression level. Error bars indicate the standard deviation (SD). *P < 0.05, **P < 0.01, ***P < 0.001