Fig. 2

SGO1-AS1 suppresses the invasive and metastatic activity of GC cells in vitro and in vivo. a-b. Transwell assay measuring the invasion of SGC7901 and BGC823 cells stably expressing SGO1-AS1 (a), MKN28 cells stably silencing SGO1-AS1 (b) and their respective control cells. c-d. Wound healing assay measuring the migratory ability of the indicated GC cells. a-d Scale bars, 150 μm. Error bars, SD from three independent experiments performed in triplicate. *P < 0.05, **P < 0.01, ***P < 0.001. e. SGC7901 cells stably expressing SGO1-AS1 or vector were intravenously injected into the tail vein of nude mice. Representative bioluminescent images and H&E-stained lung sections of the mice are shown. The number of metastatic foci was quantified. Scale bar, 200 μm. Error bars represent SD (n = 5 mice/group). Arrows or circles indicate metastatic nodules. **P < 0.01. f. Kaplan-Meier survival curve of mice in a parallel experiment. g. SGO1-AS1-overexpressing SGC7901 cells and control cells were orthotopically injected into the stomach of nude mice (10 mice per group). The mice were sacrificed 45 days later, and the tumor nodules in the abdominal cavity were examined. Representative IVIS luciferase in vivo images and bright views of livers and intestines isolated from the mice are shown. The bioluminescence signal and number of mice with metastasis were quantified. Arrows or circles indicate metastatic nodules. **P < 0.01