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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: Long noncoding RNA SGO1-AS1 inactivates TGFβ signaling by facilitating TGFB1/2 mRNA decay and inhibits gastric carcinoma metastasis

Fig. 5

SGO1-AS1 impedes TGFβ signaling, the EMT and stemness. a-b. SGO1-AS1 knockdown increased SMAD2/3 phosphorylation and SBE4 transcriptional activity, and this effect was reversed by PTBP1 knockout or SB431542 (10 μM, 24 h) as shown by Western blot (a) and luciferase reporter assays of the SBE4 promoter (b). c-d. EMT markers were measured in the indicated cells by a Western blot analysis. e. qRT-PCR analysis of EMT-TF expression in the indicated cells. f-h. Cell invasion ability (f), tumorsphere formation efficiency (g) and rates of ALDH1-positive cells (h) in the indicated cells. i. Limiting dilution xenograft formation of SGC7901 cells stably expressing SGO1-AS1 or vector (n = 5 mice per group). j. MKN28 cells with SGO1-AS1 knockdown or control were orthotopically injected into the stomach of nude mice, and the mice were treated with PBS or SB431542 (20 mg/kg body weight, i.p.) three times per week for 3 weeks (n = 6 mice per group). Tumor progression was monitored by luciferase signal intensity using an In Vivo Imaging System. Error bars indicate SDs. *P < 0.05, **P < 0.01, ***P < 0.001

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