Fig. 6

SGO1-AS1 decreases TGFβ autocrine signaling. a-b. ELISA analysis of the TGFβ1/2 protein levels in conditioned medium from cells with SGO1-AS1 knockdown (a) or overexpression (b). c. Conditioned medium from SGO1-AS1-silenced MKN28 cells enhanced SBE4 promoter luciferase activity in HEK293T cells. In contrast, SBE4 transcription in HEK293T cells treated with conditioned medium collected from the SGO1-AS1-overexpressing SGC7901 cells was downregulated compared to that in the cells treated with medium collected from the control cells. d. The cell invasion ability of MKN28 cells increased when treated with conditioned medium from SGO1-AS1-silenced MKN28 cells, but that of SGC7901 cells was decreased following the treatment with conditioned medium from SGO1-AS1-overexpressing SGC7901 cells. e. The migratory ability of MKN28 cells was increased following the treatment with conditioned medium from SGO1-AS1-silenced MKN28 cells, while that of SGC7901 cells was decreased following the treatment with conditioned medium from SGO1-AS1-overexpressing SGC7901 cells. Scale bar, 150 μm. All error bars indicate SDs. *P < 0.05, **P < 0.01, ***P < 0.001