Fig. 2

Apatinib induced G1 cell cycle arrest and apoptosis in NSCLC cells. A549 and H1299 cells were treated with indicated treatment for 48 h. a Flow cytometry assay of cell cycle distribution. b The quantitation of cell cycle distribution. The data are presented as mean ± SD (n = 3). **p < 0.01 vs. control. c Western blot analysis of G1 phase-related regulators CDK4 and Cyclin D1. d TUNEL staining of apoptosis in A549 and H1299 cells. Scar bar = 50 μm. e Western blot analysis of apoptosis-related proteins Cleaved Caspase 9, Cleaved Caspase 3, Bcl-2, and Bax expression. f Immunofluorescence staining of Cleaved Caspase 3 (Cleaved Caspase 3, green; DAPI, blue). Scar bar = 50 μm