Fig. 2

CPZ induces ATF6-α nuclear accumulation in GBM cells. ATF6-α full length (fl.) or cleaved (cl.) determination, in cytoplasmic or nuclear cell fractions, by means of western blot (images, upper left), their relative quantification (histograms, lower left) and immunofluorescence microscopy (pictures, right) in control (CTL) or CPZ-treated T98G, U-87 MG and U-251 MG anchorage-dependent GBM cells (A), TS#1, TS#83 and TS#163 (B) neurospheres and RPE-1 non-cancer cells (C). In the immunofluorescence images, ATF6-α, both fl. and cl., is represented in red, while cell nuclei are represented in blue. In western blots, H3 histone and GAPDH have been determined as nuclear and cytoplasmic markers, respectively, and used for relative quantification. Statistical significance in the histograms is referred solely to nuclear ATF6-α in CPZ-treated cells toward the respective control (CTL) (*p<0.05)